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Effect of HA14-1 on Apaf-1−/− and Apaf-1+/+ cells. Apaf-1-deficient mouse embryonic fibroblast (Apaf-1−/−) and NIH 3T3 (Apaf-1+/+) cells were treated with 50 μM HA14-1 for 24 h. Percent cell death was determined by using Trypan blue staining. To examine the role of caspases in the death of Apaf-1+/+ cells induced by HA14-1, cells were preincubated with zVAD-fmk for 2 h before the treatment of the compound.
