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. 2003 May;14(5):2163–2180. doi: 10.1091/mbc.02-05-0076

Figure 1.

Figure 1.

(A) Northern analysis of CaCDC5 expression under control of the Candida PCK1 promotor. Strains CB104 (cacdc5Δ::hisG/cacdc5Δ:: HIS1/PCK1::CaCDC5), CB102 (cacdc5Δ::hisG/CaCDC5 PCK1::CaCDC5) and RM1000 (CaCDC5/CaCDC5) were grown in SD medium for 1, 2, 4, or 7 h, or in SS medium. Total RNA, 20 μg, was analyzed with a CaCDC5-specific probe, followed by an ACT1 probe to compare RNA loading. CaCDC5 is overexpressed in SS medium and repressed in SD medium at 1–7 h of incubation, although some leakiness is present at 7 h. (B) Northern analysis of CaCDC5 expression in cells grown in YPD at 30°C without serum or at 37°C with serum for 30 and 60 min.