Figure 3.

(A) Nuclear division and septation are impaired upon repressing CaCDC5. Strains CB104 (cacdc5Δ::hisG/cacdc5Δ::HIS1/PCK1:: CaCDC5) and RM1000 (CaCDC5/CaCDC5) were grown in SS medium, washed, transferred to SD medium, and then fixed and stained with DAPI and calcofluor at various time points. Septa (small arrowheads) and fragmented DNA (arrow) were observed after 5 h. Note the absence of nuclei in the mother yeast cell after 5 h. (B) Nuclei and septa in serum-induced hyphae grown at 37°C from strain RM1000 after 1.5 and 3 h. Bar, 10 μm.