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. 2003 May;14(5):2163–2180. doi: 10.1091/mbc.02-05-0076

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Figure 6. — Hydroxyurea induces filament formation under yeast growth conditions and impairs spindle elongation. (A) Strain SC5314 was grown in YPD medium overnight and then diluted to an OD₆₀₀ of 0.4 in fresh YPD containing 200 mM HU. Cells were fixed at 6 h and stained with DAPI and calcofluor. (B) Strain CB110 (CaCDC5/CaCDC5, TUB1-GFP) was incubated in 200 mM HU for 3, 7, and 24 h. Note the similarity to filaments of CaCDC5-repressed cells (Figures 2B, 3A, and 4A). Bar, 10 μm. (C) FACS analysis demonstrating S phase and G2 phase blocks in HU-treated and CaCDC5-repressed cells, respectively. Cells of strains CB104 (cacdc5::hisGΔ/cacdc5Δ::HIS1/PCK1::CaCDC5) and RM1000 (CaCDC5/CaCDC5) were transferred from SS to SD medium, collected at the indicated time points, and processed for FACS analysis. Strain SC5314 was grown in YPD containing 200 mM HU and processed for FACS analysis. Note the fragmentation of DNA at later time points of 9 and 6 h.