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. 2003 May;14(5):2163–2180. doi: 10.1091/mbc.02-05-0076

Figure 7.

Figure 7.

Filaments produced by repression of CaCDC5 or exposure to HU are similar to hyphae in expression of a serum-induced antigen and RNA. (A) Serum-induced hyphae from strain SC5314, filaments from strain CB104 (cacdc5Δ::hisG/cacdc5Δ:: HIS1/PCK1::CaCDC5) depleted of CaCdc5p for 7 h, and filaments from strain SC5314 incubated in HU for 24 h were fixed and processed for immunolocalization of MAb16B1-F10. Bar, 10 μm. (B) Northern analysis demonstrating the filament-induced expression of RNA normally induced by the hyphal-generating conditions of serum and high temperature. Total RNA, 20 μg, from strain SC5314 incubated in YPD with 200 mM HU for 6 h, SC5314 without HU, CB104 (cacdc5::hisGΔ/cacdc5Δ:: HIS1/PCK1::CaCDC5) grown in SD medium for 24, 7, and 4 h, and CB102 (cacdc5Δ::hisG/CaCDC5 PCK1:: CaCDC5) grown in SD medium for 4 h was hybridized with probes specific for HWP1, DDR48, and ACT1 as a loading control.