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. 2003 May;14(5):2163–2180. doi: 10.1091/mbc.02-05-0076

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Copyright © 2003, The American Society for Cell Biology

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Figure 8. — Filamentous growth in cells depleted of CaCdc5p or exposed to HU require CaCdc35p, but not Efg1p and Cph1p. Cells from strains CB108 (cacdc5Δ::hisG/MET:: CaCDC5-URA3), CB305 (cacdc5Δ::hisG/MET:: CaCDC5-URA3 cph1Δ::hisG/cph1Δ::hisG efg1Δ::hisG/efg1Δ::hisG) and CB303 (cacdc5Δ::hisG/MET:: CaCDC5-URA3 cdc35Δ::hisG/cdc35Δ::hisG) were incubated in SD medium–methionine for overexpression of MET:: CaCDC5, or SD medium + methionine and cysteine to repress MET:: CaCDC5 expression for 7 h. Cells from strains SC5314 (+/+), HLC54 (cph1Δ::hisG/cph1Δ::hisG efg1Δ::hisG/efg1Δ::hisG -URA3-hisG) and CR216 (cdc35Δ::hisG/cdc35Δ::hisG-URA3-hisG) were grown in SD medium, diluted to an OD₆₀₀ of 0.4 in fresh medium containing 200 mM HU, and incubated for 7 h. Cells were fixed in 70% EtOH before collection of images. Bar, 10 μm.