Table 1.
Candida albicans strains and oligos used in this study
| Genotype | Source | |
|---|---|---|
| Strain | ||
| SC5314 | CaCDC5/CaCDC5 URA3/URA3 HIS1/HIS1 | Fonzi and Irwin (1993) |
| CA14 | ura3Δ::imm434/ura3Δ::1 imm434 | Fonzi and Irwin, 1993 |
| RM1000 | ura3Δ::imm434/ura3Δ::1 imm434 his1Δ::hisG/his1Δ::hisG | Negredo et al. (1997) |
| JCK19 | cph1Δ::hisG/cph1Δ::hisG ura3Δ::imm434/ura3Δ::1 imm424 | Lo et al., (1997) |
| HLC52 | efg1Δ::hisG/efg1Δ::hisG URA3-hisGΔ::imm434/ura3Δ::1 imm434 | Lo et al., 1997 |
| HLC54 | HLC52 cph1Δ::hisG/cph1Δ::hisG | Lo et al., 1997 |
| HLC69 | cph1Δ::hisG/cph1Δ::hisG efg1Δ::hisG/efg1Δ::hisG ura3Δ:: imm434/ura3Δ::1 imm434 | Lo et al., 1997 |
| CR216 | CAI4 cdc35Δ::hisG-URA3-hisG/cdc35Δ::hisG | Rocha et al., 2001 |
| CR276 | cdc35Δ::hisG/cdc35Δ::hisG | Rocha et al., 2001 |
| CB102 | cacdc5Δ::hisG/CaCDC5 PCK1::CaCDC5-URA3 | This study |
| CB104 | cacdc5Δ::hisG/cacdc5Δ::HIS1 PCK1::CaCDC5-URA3 | This study |
| CB105 | cacdc5Δ::hisG/cacdc5Δ::HIS1 PCK1::CaCDC5-hisG | This study |
| CB108 | CAI4 cacdc5Δ::hisG/MET::CaCDC5-URA3 | This study |
| CB109 | cacdc5Δ::hisG/CaCDC5 MET::(URA3) | This study |
| CB110 | RM1000 (TUB1-GFP-URA3) | This study |
| CB112 | CB105 (TUB1-GFP-URA3) | This study |
| CB113 | CB105 (CDC12-GFP-URA3) | This study |
| CB115 | RM1000 (PCK1::CaCDC5-GFP-URA3) | This study |
| CB116 | RM1000 (CaCDC5-GFP-URA3) | This study |
| CB303 | CB108 cdc35Δ::hisG/cdc35Δ::hisG | This study |
| CB305 | CB108 cph1Δ::hisG/cph1Δ::hisG efg1Δ::hisG/efg1Δ::hisG | This study |
| CB400 | RM1000 (pRM100 URA3+, HIS1+) | This study |
| CB401 | CB102 (pCB102 HIS1+) | This study |
| Oligos | ||
| CB1F (KpnI) | TCGAGCAGGACCAATTGC | |
| CB1R (PstI) | CTGTGGTGGGTGAAGCGA | |
| CB3F (BamHI) | CGAAGCGCCGACATATCA | |
| CB3R (BamHI) | CATGAAAATGTTCCGG | |
| CB4F (Not1) | CGAAGCGCCGACATATCA | |
| CB4R (BglII) | CATGAAAATGTTCCGG | |
| CB5F (KpnI) | TGATATGTCGGCGCTTCG | |
| CB5R (KpnI) | GCTTTGCAAAATGCATGTTCC | |
| CB6F (PstI) | TGATATGTCGGCGCTTCG | |
| CB6R (BamHI) | GGAAATTCACTGGCATCAAC | |
| CB50F (BglII) | CCAAAGGGAGGAGAAGAA | |
| CB50R (SacI) | ACAAATAAATAAATCGCTCGG | |
| CB51F (SpeI) | GTAGTATGGTTGATCGTGTTC | |
| CB51R (XhoI) | ATATTCTTCTTCTTCTTCAGGGAAAGAATCAGT | |
| CB43F (SpeI) | AGACAACGCTACTTGATATTT | |
| CB43R (XhoI) | AGCTTCTTTAAATGCTTTTTTC | |
| CB44F (XhoI) | ATGAGTAAAGGAGAAGAACTTTTC | |
| CB44R (SpeI) | TTATTTGTATAGTTCATCCATGCC |