Table 2.
Proportion of cells (%) in a single, budded, or elongated morphology upon incubation in SD medium
| Single
|
Buddeda
|
Elongatedb
|
||||
|---|---|---|---|---|---|---|
| Hours | CB104 | RM1000 | CB104 | RM1000 | CB104 | RM1000 |
| 0 | 88.4 | 99.5 | 11.6 | 0.5 | 0 | 0 |
| 3 | 2.0 | 4.4 | 26.0 | 83.2 | 72.0 | 10.6 |
| 5 | 0 | 7.9 | 0 | 91.1 | 100 | 0 |
| 8 | 0 | 47.0 | 2.5 | 53.0 | 97.5 | 0 |
Cells from strains CB104 (cacdc5Δ::hisG/cacdc5Δ::HIS1/PCK1p::CaCDC5) and strain RM1000 (CaCDC5/CaCDC5) were grown at 30°C in SD medium and fixed after 3, 5, and 8 h. More than 200 cells were counted in each time point.
a
Budded cells include those cells containing a bud equal to or smaller than the mother yeast cell in size and budded pseudohyphal cells.
b
Elongated cells contain an elongated secondary extension with a length greater than the diameter of the mother yeast cell or equal to the mother cell but more narrow.