Table 3.
Proportion of cells demonstrating nuclear division and positioning upon incubation in SD medium or serum
| No. of nuclei
|
Position of nuclei
|
|||||||
|---|---|---|---|---|---|---|---|---|
| Strain | 1 | 2 | 3 | >3 | Fraga | Mother | Mother and tube | Tube |
| Hours in SD | ||||||||
| CB104 | ||||||||
| 0 (n = 242) | 100 | 0 | 0 | 0 | 0 | 100 | 0 | 0 |
| 3 (n = 212) | 92.0 | 8.0 | 0 | 0 | 0 | 68.0 | 8.0 | 24.0 |
| 5 (n = 319) | 55.0 | 45.0 | 0 | 0 | 0 | 1.0 | 36.7 | 62.4 |
| 8 (n = 197) | 2.5 | 65.5 | 3.6 | 0 | 28.4 | 0 | 1.5 | 70.0 |
| RM1000b | ||||||||
| 0 (n = 200) | 100 | 0 | 0 | 0 | 0 | — | — | — |
| 3 (n = 236) | 88.6 | 11.4 | 0 | 0 | 0 | — | — | — |
| 5 (n = 238) | 89.5 | 10.5 | 0 | 0 | 0 | — | — | — |
| 8 (n = 225) | 86.2 | 13.8 | 0 | 0 | 0 | — | — | — |
| Hours in serum at 37°C | ||||||||
| RM1000 | ||||||||
| 0 (n = 100) | 100 | 0 | 0 | 0 | 0 | 100 | 0 | 0 |
| 1.5 (n = 106) | 52.0 | 48.0 | 0 | 0 | 0 | 33.3 | 34.5 | 32.2 |
| 3.0 (n = 59) | 0 | 20 | 58 | 22.0 | 0 | 0 | 100 | 0 |
Values are expressed as percentage. Cells from strain CB104 or RM1000 were incubated in SD medium at 30°C for the indicated times, fixed, and stained with DAPI. Cells from strain RM1000 were incubated in SD medium plus 10% serum at 37°C for the indicated time points and fixed and stained with DAPI.
a
Fragmentation of nuclei prevented an accurate quantification of the total number of nuclei per cell after 8 h of repression.
b
Cells containing 2 nuclei were doublets.