Table 5.
Spindle elongation and cell morphology in cells depleted of CaCdc5p, exposed to HU or treated with serum
| Spindle elongation | Cell Morphology | ||||||
|---|---|---|---|---|---|---|---|
|
|||||||
| Strain | Hours in SD | S/early G2a | G2/Mb | Mc | Single or small bud | Large Bud | Elongated |
| CB112 | 3 (n=226) | 92.0 | 5.8 | 2.2 | 31.0 | 11.0 | 58.0 |
| 4 (n=267) | 84.0 | 9.7 | 6.3 | 0 | 17.0 | 83.0 | |
| 7 (n=186) | 66.0 | 28.0 | 5.9 | 0 | 0 | 100 | |
| CB110 | |||||||
| 3 (n=236) | 87.0 | 1.8 | 11.2 | 63.0 | 24.0 | 13 | |
| 4 (n=180) | 85.0 | 4.6 | 10.4 | 76.0 | 18.9 | 5.6 | |
| 7 (n=190) | 89.5 | 1.0 | 9.5 | 87.8 | 12.2 | 0 | |
| Hours in HU | |||||||
| CB110 | 3 (n=274) | 97.7 | 2.3 | 0 | 0 | 5.4 | 94.6 |
| 7 (n=397) | 81.0 | 18 | 1.0 | 0 | 0 | 100 | |
| Hours in serum at 37°C | |||||||
| CB110 | 3 (n= 75) | 81.3 | 10.7 | 8.0 | 0 | 0 | 100 |
Values are expressed as percentages.
a
Unseparated spindle pole bodies and early spindles represented by a spot or two spots side by side.
b
Short bar spindles equivalent to 3 to 4 spots in length.
c
Extended mitotic spindles, greater than 4 spots in length.