Figure 4.

Isoform dependence of the interaction of CnBP-B with Cn. (a) Amino acid sequence of peptides corresponding to the CnBP-B region from the four known NFAT isoforms. NFATcΔ is a truncated version of the NFATc peptide. The “core” binding sequence is boxed, and the prolines that are critical for binding, as judged from Fig. 3b, are highlighted in gray. (b) NFAT peptides derived from the various isoforms were fused to the C terminus of GST and used in Cn pull-down assays. (c) The same NFAT baits as in b were used to pull down Cn in the presence of the NFATc CnBP-B peptide as competitor (50, 100, and 300 μM concentrations). (d) Quantification of the results in c analyzed by scanning and macbas.