Figure 3.

Sp22D polypeptides detected by immunoblotting. (A) Hemolymph (he) of 4-day-old mosquitoes of both susceptible (s) and refractory (r) strains; (Ad) whole extracts of adult mosquitoes. (B) Early pupal (EP), late pupal (LP), and adult (Ad) extracts. (C) Supernatants (conditioned medium) of cell lines 3A and 3B; (he*) hemolymph of freshly emerged adults. (D) RNA blot displaying a single ca. 4.5-kb transcript in cell lines 3A and 3B. (E) Schematic representation of the Sp22D protein indicating the region against which a specific rat antiserum was raised Ab(Sp22D), the potential proteolytic cleavage sites (arrowheads), and the possible glycosylation sites (*).