FIG. 1.

PAGE of ER purified from the newly isolated C. magnoliae. (A) Native PAGE. Lane 1, cell extract; lane 2, DEAE ion-exchange fraction; lane 3, Sephadex G-100 fraction; lane 4, Cibacron Blue 3GA affinity fraction; lane 5, preparative electrophoresis fraction. (A-a) Activity staining after native PAGE. (B) SDS-PAGE. The enzyme solution was run on a 10% (wt/vol) polyacrylamide slab gel as described in Materials and Methods. The standard proteins (Bio-Rad) used for the estimation of molecular mass were phosphorylase B (113 kDa), bovine serum albumin (92 kDa), ovalbumin (52.3 kDa), carbonic anhydrase (39.2 kDa), soybean trypsin inhibitor (28.9 kDa), and lysozyme (21 kDa).