FIG. 5.
Graphical analysis of the inhibition of C. magnoliae ER by erythritol. The effects of increasing erythritol (product) concentration on the apparent Km and Vmax values for erythrose and NADH were examined. Analysis of these data by double-reciprocal plots indicated that erythritol inhibited ER noncompetitively with respect to erythrose (A) and NADH (B). In panel C, the secondary plots for noncompetitive inhibition with erythrose and NADH are shown. The erythritol product binds to ER with a Ki of 276 mM.