TABLE 3.
Substrate and coenzyme specificity of C. magnoliae ERa
| Substrate | Km (mM) | kcat (s−1) | kcat/Km (mM−1 s−1) |
|---|---|---|---|
| p-Nitrobenzaldehyde | 0.13 | 1.8 | 14 |
| p-Carboxybenzaldehyde | 0.53 | 1.1 | 2.1 |
| Benzaldehyde | 0.59 | 1.2 | 2.1 |
| d-Glyceraldehyde | 3.8 | 6.6 | 1.7 |
| d-Erythrose | 7.9 | 5.7 | 0.73 |
| d-Xylose | 36 | 2.3 | 0.064 |
| d-Arabinose | 42 | 1.4 | 0.034 |
| d-Ribose | 61 | 1.3 | 0.022 |
| d-Galactose | 70 | 0.70 | 0.010 |
| d-Glucose | 76 | 1.3 | 0.017 |
| NADH | 0.013 | 5.8 | 450 |
| NADPH | 0.12 | 0.66 | 5.5 |
a
The purified enzymes were assayed under standard assay conditions with various substrates. Each value represents the mean of triplicate measurements and varied from the mean by not more than 10%.