TABLE 3.

Tested cooling rates (CR) for Entodinium caudatum from the equilibration temperature (ET) to the holding temperature (HT), set at −33°C^a

Curve 3		Curve 3a		Curve 3b
ET → HT (°C)	CR (°C/min)	ET → HT (°C)	CR (°C/min)	ET → HT (°C)	CR (°C/min)
25 → −6*	12.4	26 → −5*	6.8	26 → −5.5*	13
−2.5** → −4	0.6	−3** → −4	0.4	−2.5** → −4	0.7
−4 → −8	2.6	−4 → −8	1.0	−4 → −8	7.8
−8 → −19	4.2	−8 → −19	5.2	−8 → −19	7.7
−19 → −23	2.1	−19 → −23	3.0	−19 → −23	7.8
−23 → −25.5	1.7	−23 → −25.5	2.1	−23 → −25.5	3.6
−25.5 → −29	2.5	−25.5 → −29	1.0	−25.5 → −29	3.9
−29 → −30.5	1.8	−29 → −30.5	0.8	−29 → −30.5	2.3
−30.5 → −33	1.7	−30.5 → −33	0.7	−30.5 → −33	2.1

^aTimes from ET to extracellular ice nucleation temperature (∗) were 3.2 (curve 3), 2.5 (curve 3a), and 2.0 (curve 3b) min; the times from after extracellular ice nucleation temperature (∗∗) to HT were 10.8 (curve 3), 12.6 (curve 3a), and 4.6 (curve 3b) min; and the total times from ET to HT were 14 (curve 3), 15.1 (curve 3a), and 6.6 (curve 3b) min; (see Fig. 1). ET, temperature indicated by the thermocouple in the tube containing ciliates (after the equilibration time) just before the freezing cycle.