FIG. 2.

Effect of the expression of Msn2p- and Msn4p-regulated genes, encoding antioxidant enzymes and heat shock proteins, on yeast resistance to 2,4D. Growth susceptibilities to 2,4-D for wild-type (wt) strain BY4741 and 12 mutants with the indicated genes deleted were tested by comparing their growth curves in the absence (0 mM) or presence (0.62 mM) of 2,4-D. The growth curves shown are average values from at least three independent growth experiments. Cell suspensions used to prepare the inocula to obtain an initial OD₆₀₀ of 0.1 ± 0.01 were cultivated in the absence of herbicide until a standardized OD₆₀₀ of 0.5 ± 0.05 was attained, in mid-exponential phase.