FIG. 7.

Time course variation in the accumulation of ¹⁴C-labeled 2,4-D in cells of the wild-type (•) and the Δspi1 mutant (○) strains, in the absence of glucose (− Glu) and its subsequent expulsion after a glucose pulse (+ Glu) (arrow). Cells were previously adapted to the herbicide and were harvested at mid-exponential phase (culture OD₆₀₀ = 1 ± 0.1) of cultivation in minimal medium supplemented with 0.3 mM 2,4-D at pH 3.5. The corresponding growth curves are shown in Fig. 3. Accumulation values are representative of the several experiments.