FIG. 3.

Growth of different yeast transformants in l-arabinose medium. Yeast transformants were pregrown in synthetic medium with 2% glucose, washed twice, and inoculated in synthetic medium with 2% l-arabinose at an initial OD₆₀₀ of about 0.2. Cells were grown aerobically at 30°C in shaker flasks on a rotary shaker. Growth was monitored by measuring the OD₆₀₀s of the cultures. All growth tests were performed two to four times, with similar results. Representative results are shown here. The strains are JBY25-4M (▪) (A and B); JBY24-4V (♦), JBY24-4P (∗), and JBY25-4P (▴) (A); and JBY25-3M (♦), JBY24-3T (▴), and JBY24-4M (∗) (B). The doubling time of JBY25-4M in the exponential growth phase was 7.9 h. The various plasmid compositions of the transformants are indicated on the right side of the growth curves. wt, wild-type background (JBY24); mut, mutant background (JBY25).