FIG. 4.

Ethanol production from l-arabinose and glucose. The yeast transformants JBY25-4M and JBY24-4V were pregrown in synthetic medium with 2% glucose or 2% l-arabinose until they reached an OD₆₀₀ of about 2, washed twice, and incubated at 30°C in synthetic medium with 2% glucose or 2% l-arabinose under oxygen-limiting conditions at an OD₆₀₀ of about 15 to 20. Ethanol concentrations in the culture supernatants were determined at various time points. Representative results of at least two independent experiments for each strain are shown. •, JBY24-4V with glucose; ♦, JBY24-4V with l-arabinose; ▴, JBY25-4M with glucose; ▪, JBY25-4M with l-arabinose. EtOH, ethanol.