FIG. 3.

Heating times at 75 to 93°C required to prevent growth from 10⁶ spores of C. botulinum type E strain Beluga in rainbow trout and whitefish media. ⧫, growth observed in rainbow trout medium within 90 days at 30°C, ◊, no growth observed in rainbow trout medium; •, growth observed in whitefish medium within 90 days at 30°C, ○, no growth observed in whitefish medium; - - - -, 6D heat treatments (i.e., heat treatments proposed to eliminate 10⁶ nonproteolytic spores) recommended by the Advisory Committee on the Microbiological Safety of Foods (1); - - - - - -, 6D heat treatments recommended by the European Chilled Food Federation (17).