FIG. 2.

Schematic representation of the specific crtYB gene inactivation approach by single (A) and double (B) crossover. Prior to the introduction in X. dendrorhous by electrotransformation, the plasmids pPR16 and pPR19F were linearized with the endonucleases BstXI and EcoRI, respectively. SI, site of insertion (the insert was a blunted EcoRI fragment in a blunted BstXI site) (Table 1).