Figure 6.

Neuronal-specific expression of NIX1 mRNA. (A) Northern blot analysis of poly(A)⁺ RNA isolated from the indicated mouse tissues and Neuro2A cells. A total of 15.0 μg of poly(A)⁺ RNA was loaded in each lane. Hybridization using a ³²P-labeled NIX1 cDNA probe identified a mRNA of approximately 1.4 kb. (B) In situ hybridization of NIX1 in mouse brain. NIX1 mRNA was exclusively found in neuronal cells. Digoxigenin-labeled anti-sense RNA was used to localize NIX1 mRNA in a coronal section to the dentate gyrus of the hippocampus (d.g.) and to the central nucleus of the amygdala (c.a.n.). NIX1 mRNA is also found in thalamic and hypothalamic regions, and in several brainstem nuclei.