TABLE 1.
Results of sensitivity analysis with synthetic data for which the parent cell mean fluorescence intensity was 2.3 and the standard deviation was variable
| Standard deviation | % in the parent population/% in the daughter populationa | % Error
|
|
|---|---|---|---|
| AFI method | AG method | ||
| 0.079 | 10/90 | 5.4 | 0.0 |
| 25/75 | 11.0 | 0.0 | |
| 50/50 | 0.0 | 0.0 | |
| 0.146 | 10/90 | 2.7 | 0.0 |
| 25/75 | 3.9 | 0.0 | |
| 50/50 | 0.0 | 0.0 | |
| 0.204 | 10/90 | 1.6 | 0.0 |
| 25/75 | 2.2 | 0.0 | |
| 50/50 | 0.0 | 0.0 | |
| 0.146b | 50/50 | 5.1 | 11.5 |
a
Percentages in each generation that were utilized to construct the fluorescence intensity distribution with the mean and standard deviation. The data were then fit with either the AFI or the AG method, and the error was reported.
b
Average data for 10 calculations when random noise was added to each data point and the mean fluorescence intensity of the parent and daughter generations.