TABLE 3.

Characteristics of selected strains of V. parahaemolyticus

Class	Strain no.	Sample	Origina	Date of isolation	Serotype	toxR gene	tdh geneb	GS-PCR	AP-PCR patternc obtained with:		PFGE profiled
									Primer 2	Primer 4
Japanese environmental samples	VP1152	Rock oyster	N	24 July 2001	O3:K6	+	+	+	a	i	H
	VP1153	Rock oyster	N	24 July 2001	O3:K6	+	+	+	a	i	F
	VP1219	Rock oyster	N	7 Aug. 2001	O3:K6	+	+	+	a	i	F
	VP1280	Rock oyster	N	30 Aug. 2001	O3:K6	+	+	+	a	i	E
	VP1281	Rock oyster	N	30 Aug. 2001	O3:K6	+	+	+	a	i	E
	VP1282	Rock oyster	N	30 Aug. 2001	O3:K6	+	+	−	c	ii	NDe
	1001A44	Hen clamf	N	17 Sep. 2001	O3:K6	+	−	+	b	i	J
	1001A46	Hen clamf	N	17 Sep. 2001	O3:K6	+	−	+	b	i	K
	VPF01-13	Hen clam	C	6 Aug. 2001	O3:K6	+	+	+	a	i	ND
	VP80	Hen clam	C	20 Aug. 2001	O3:K6	+	+	+	a	i	F
	VPF01-5	Short-neck clam	C	16 July 2001	O3:K6	+	+	+	a	i	ND
	19-13	Hen clam	U	14 Aug. 2001	O3:K6	+	+	+	a	i	F
	VPF00-18	Short-neck clam	C	16 Oct. 2000	O3:K6	+	+	+	a	i	G
	VPF00-10	Short-neck clam	C	2 Oct. 2000	O3:K6	+	+	+	a	i	E
	APCC VP 00157	Sediment of river N	N	July 2001	O1:K25	+	+	+	a	i	O
	APCC VP 00190	Sediment of river N	N	Aug. 2001	O4:K68	+	+	+	a	i	P
	APCC VP 00030	Sediment of river A	N	Dec. 2000	O3:K6	+	−	+	b	i	L
	APCC VP 00031	Sediment of river K	N	Dec. 2000	O3:K6	+	−	+	b	i	M
	APCC VP 9810	Sediment of river N	N	Aug. 1998	O3:K6	+	+	+	a	i	I
Reference strainsg	FIHES98V1-32-4	Clinical specimen	Japan	1998	O3:K6	+	−	+	b	i	A
	JKY-VP6	Clinical specimen	Japan	1998	O3:K6	+	+	+	a	i	B
	VP-2	Clinical specimen	Korea	1998	O3:K6	+	+	+	a	i	C
	BE98-2062	Clinical specimen	USA	1998	O3:K6	+	+	+	a	i	D
	KX-V225	Clinical specimen	Int.travel.	1996	O3:K6	+	+	+	a	i	E
	AN-8373	Clinical specimen	Bangladesh	1998	O3:K6	+	+	+	a	i	E
	VP-81	Clinical specimen	India	1996	O3:K6	+	+	+	a	i	E
	DOH958-15	Clinical specimen	Taiwan	1997	O3:K6	+	+	+	a	i	E
	97LVP2	Clinical specimen	Laos	1997	O3:K6	+	+	+	a	i	E
	VP-47	Clinical specimen	Thailand	1998	O3:K6	+	+	+	a	i	E
	VP-67	Clinical specimen	Thailand	1999	O3:K6	+	+	+	a	i	E
	4917	Clinical specimen	Thailand	1999	O1:K25	+	+	+	a	i	N
	5095	Clinical specimen	Thailand	1999	O4:K68	+	+	+	a	i	P

^aN, northern Japan; C, central Japan; U, unknown; Int. travel., International traveler to Thailand.

^bAbility to produce TDH was confirmed by detection of TDH by reversed-phase passive latex agglutination test.

^cThe patterns are shown in Fig. 3.

^dThe profiles are shown in Fig. 4A.

^eND; not determined because of DNA degradation.

^fFrom the same sample.

^gMatsumoto et al. (17) and M. Nishibuchi (unpublished data).