Agarose gel electrophoresis of RT-PCR products amplified from serial dilutions of S. cerevisiae RNA extracts. Lane M, 100-bp DNA ladder; lanes 1 through 7, amplification from 25 ng, 15 ng, 5 ng, 250 pg, 150 pg, 50 pg, and 25 pg of S. cerevisiae RNA in the RT-PCR mixture, respectively; lane 8, amplification from 20 ng of S. cerevisiae DNA; lane 9, negative control.