FIG. 3.

Real-time RT-PCR assays of serial dilutions of mixed cultures of yeasts and molds. (A) Increase in fluorescence intensity with the number of PCR cycles. Fungal contaminants were present at 7 (□), 6 (♦), 5 (Δ), 4 (▪), 3 (⋄), 2 (▴), and 1 (○) log₁₀ CFU ml⁻¹. The straight horizontal line indicates the threshold value. (B) C_T values plotted against the log₁₀ CFU ml⁻¹ derived from the plate count; three repetitions for each dilution are indicated.