Figure 2.

Scanning mutagenesis of the region downstream of the β-globin initiator element indicates the presence of a downstream core promoter element. (A) Representative in vitro transcriptional analysis of triplet mutations from +10 to +45 of the β-globin promoter. The sequence of the wild-type β-globin downstream region is indicated underneath the autoradiograph. (B) Graphical presentation of the scanning mutagenesis. Bars indicate the mean of three experiments with standard deviations indicated by error bars.