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Degradation control assays indicate that DCE mutants do not significantly affect RNA stability. (A) Results of an in vitro transcription assay to analyze RNA stability of various DCE mutants at times after the addition of α-amanitin. (B) Graph of primer extension signal intensity relative to the 0 min data point. Each mutant template was normalized to a relative transcription level of 1. The data points in the graph are the mean of three experiments.
