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. 2000 Jun 6;97(13):7172–7177. doi: 10.1073/pnas.120181197

Figure 4.

Figure 4

The DCE functions in a heterologous context to increase transcription from a βInr-dependent template. Lane 1 is primer extension analysis of an in vitro transcription assay using the Sp1/βInr described previously (24). The correctly initiated transcript is indicated by the arrow to the left of lane 1 (24). Lane 2 is an in vitro transcription assay using the longer Sp1/βInrDCE template extending to +40. In two experiments, it was expressed 3× and 8× higher than the Sp1/βInr template in lane 1. The template in lane 3 contains the β-thalassemia mutation at +22, and lane 4 contains a double point mutation in the βInr element (20). These templates are otherwise identical to Sp1/βInrDCE in lane 2. The arrow to the right of lane 4 indicates the position of the correctly initiated transcript.