FIG. 2.

SSCP analysis of PCR-amplified 16S rRNA gene fragments from bacterial communities of a curd sample. (A) V3 region; (B) V2 region, forward primer labeled with NED; (C) high GC% gram-positive community; (D) V2 region, reverse primer labeled with HEX. D1 shows the SSCP-PCR product that was not diluted, and D2 shows the SSCP-PCR product that was diluted (1/5) in sterile water. y axis, fluorescence; x axis, elution in scans (unit of GeneScan software). The positions and labeling of peaks discussed in Tables 2 to 4 and in the text are indicated.