TABLE 5.
Repeatability of the SSCP analysis
| Peak | Mean % repeatability of SSCP analysisa for:
|
|||||
|---|---|---|---|---|---|---|
| Sample 1
|
Sample 2
|
Sample 3
|
||||
| Mean | SD | Mean | SD | Mean | SD | |
| 1 | 0.12 | 0.02 | 0.25 | 0.09 | ||
| 2 | 0.07 | 0.02 | 0.37 | 0.23 | 2.39 | 0.32 |
| 3 | 1.24 | 0.20 | 1 | 0.19 | 1.19 | 0.10 |
| 4 | 0.71 | 0.16 | 1.46 | 0.36 | 0.81 | 0.08 |
| 5 | 1.26 | 0.23 | 1.12 | 0.36 | 2.16 | 0.12 |
| 6 | 0.52 | 0.18 | 0.68 | 0.02 | ||
| 7 | 0.61 | 0.09 | 0.57 | 0.06 | ||
| 8 | 0.38 | 0.12 | ||||
| 9 | 0.71 | 0.05 | ||||
| 10 | 91.7 | 1.29 | 90.57 | 1.51 | 86.13 | 0.87 |
| 11 | 4.9 | 0.65 | 3.28 | 0.08 | 5.81 | 0.48 |
a
That is, extraction of nucleic acid, PCR amplification, electrophoresis, and SSCP pattern treatment.