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Survival of mycobacteria within A. castellanii at 15°C. Time zero represents 24 h after infection, immediately after treatment with amikacin. Each bar represents the mean of triplicate wells, and error bars show the standard error of the mean. Approximately 3 × 106 amoebae were added to each monolayer. (A) Survival in the presence of amoebae. (B) Survival in PAS.
