TABLE 1.
Results of DGGE, ARDRA, sequence analysis and presumptive phylogenetic affiliations of bands
| Sample and clone type in DGGE | No. of clones matching band type | ARDRAa | Clone code | Closest relative (sequence length, ∼500 bp) | % Similarity | Taxon |
|---|---|---|---|---|---|---|
| Rhizosphere, 20 days | ||||||
| R20A | 3 | 3 | r20-61 | Alternaria cheiranthi | 100 | Ascomycetes-Pleosporales |
| R20B | 29 | 1 | r20-71 | Paraphaeosphaeria quadriseptatab | 99.4 | Ascomycetes-Pleosporales |
| 24 | r20-21 | Paraphaeosphaeria quadriseptatab | 99.5 | Ascomycetes-Pleosporales | ||
| 2 | r20-10 | Setosperia monoceras | 98.8 | Ascomycetes-Pleosporales | ||
| 1 | r20-12 | Setosperia monocerasb | 98.8 | Ascomycetes-Pleosporales | ||
| 1 | r20-111 | Phaeospheria nodorum | 96.9 | Ascomycetes-Pleosporales | ||
| Rhizosphere, 90 days | ||||||
| R90A | 4 | 1 | r90-1 | Gibberella pullcaris | 99.83 | Ascomycetes-Hypocreales |
| 1 | r90-63 | Gibberella pullcaris | 99.65 | Ascomycetes-Hypocreales | ||
| 1 | r90-78 | Gibberella pullcaris | 99.65 | Ascomycetes-Hypocreales | ||
| 1 | r90-85 | Raciborskiomyces longisetosumb | 99.9 | Ascomycetes-Pleosporales | ||
| R90B | 8 | 8 | r90-16 | Paraphaeosphaeria quadriseptatab | 99.2 | Ascomycetes-Pleosporales |
| R90C | 8 | 1 | r90-67 | Chaetomlum globosum | 97.2 | Ascomycetes-Sordariales |
| 1 | r90-74 | Raciborsklomyces longisetosum | 96.0 | Ascomycetes-Pleosporales | ||
| 5 | r90-4 | Raciborsklomyces longisetosumb | 99.3 | Ascomycetes-Pleosporales | ||
| 1 | r90-75 | Raciborsklomyces longisetosumb- Cladosporium cladosporoidesb | 100 | Ascomycetes-Pleosporales | ||
| R90D | 4 | 1 | r90-26 | Sporidiobolus johnsonii-S. salmonicolor | 100 | Basidiomycetes-Sporidiales |
| 1 | r90-5 | Sporidiobolus johnsonii-S. salmonicolor | 100 | Basidiomycetes-Sporidiales | ||
| 1 | r90-71 | Bullera unicab | 97.2 | Basidiomycetes-Filobasidiales | ||
| 1 | r90-95 | Sporidiobolus johnsonii-S. salmonicolor | 98.6 | Basidiomycetes-Sporidiobolaceae | ||
| R90E | 7 | 1 | r90-2 | Cryptococcus magnusb-Filobasidium uniguttulatumb | 99.8 | Basidiomycetes-Filobasidiales |
| 3 | r90-69 | Cryptococcus luteolus | 97.5 | Basidiomycetes-Filobasidiales | ||
| 1 | r90-13 | Cryptococcus luteolus | 99.3 | Basidiomycetes-Filobasidiales | ||
| 1 | r90-29 | Cryptococcus luteolus | 99.3 | Basidiomycetes-Filobasidiales | ||
| 1 | r90-66 | Cryptococcus luteolus | 99.8 | Basidiomycetes-Filobasidiales | ||
| R90F | 7 | 1 | r90-61 | Bullera oryzaeb | 99.7 | Basidiomycetes-Filobasidiales |
| 5 | r90-70 | Bullera hannae | 99.5 | Basidiomycetes-Filobasidiales | ||
| 1 | r90-87 | Bullera hannae | 99.5 | Basidiomycetes-Filobasidiales | ||
| Soil, 20 days | ||||||
| S20A | 5 | 1 | s20-5 | Aspergillus nomius | 99.8 | Ascomycetes-Eurotiales |
| 1 | s20-72 | Aspergillus terreus | 99.7 | Ascomycetes-Eurotiales | ||
| 1 | s20-2 | Blonectria ochroleuca | 99.6 | Ascomycetes-Hypocreales | ||
| 1 | s20-63 | Mortierella wolfil | 99.1 | Zygomycetes-Mucorales | ||
| 1 | s20-105 | Alternaria cheiranthi | 99.83 | Ascomycetes-Pleosporales | ||
| S20B | 4 | 4 | s20-67 | Cucurbita berberidisb | 99.5 | Ascomycetes-Pleosporales |
| S20G | 4 | 1 | s20-8 | Mortierella chlamydospora | 96.87 | Zygomycetes-Mucorales |
| 1 | s20-12 | Mortierella chlamydospora | 99.1 | Zygomycetes-Mucorales | ||
| 1 | s20-19 | Mortierella chlamydospora | 99.1 | Zygomycetes-Mucorales | ||
| 1 | s20-108 | Mortierella chlamydospora | 97.4 | Zygomycetes-Mucorales | ||
| S20H | 4 | 3 | s20-62 | Chaetomium globosum | 99.7 | Ascomycetes-Sordariales |
| 1 | s20-71 | Cladosporium cladosporoidesb | 100 | Ascomycetes-Pleosporales |
a
Number of clones grouped in each OTU generated by ARDRA analysis.
b
Sequence length, ∼1,600 bp.