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. 2003 Jul;69(7):4183–4189. doi: 10.1128/AEM.69.7.4183-4189.2003

FIG. 1.

FIG. 1.

Two percent agarose gel electrophoresis analysis of amplicons before and after simultaneous digestion with restriction enzymes DraI and VspI. Lanes 1, 3, 5, 7, and 9, undigested PCR product from C. parvum, C. muris, C. baileyi, C. felis, and C. meleagridis, respectively; lanes 2, 4, 6, 8, and 10, fragmented PCR product from C. parvum (222, 112, and 104 bp), C. muris (319 and 112 bp), C. baileyi (128, 112, 104, and 84 bp), C. felis (189, 112, 104, and 50 bp), and C. meleagridis (171, 112, 104, and 47 bp), respectively. Lanes M, 100-bp DNA ladder. In this figure, bands of sizes 112 and 104 bp comigrate and appear as a single band. This does not affect the interpretation of the RFLP analysis nor the discrimination among the five species.