TABLE 1.
Cryptosporidium spp. and genotypes determined by RFLP of the amplicon defined by the CPB-DIAGR/F primers after digestion with the enzymes VspI, DraI, and DdeI
| Cryptosporidium spp. and genotypes (total amplicon length in bp) | Amplicon length(s) (bp) as determined with:
|
GenBank accession no. | ||
|---|---|---|---|---|
| VspI | DraI | DdeI | ||
| C. parvum 1 (438) | 222, 104, 112 | None | 204, 68, 166 | L16997 |
| C. parvum 2 (435) | 219, 104, 112 | None | 201, 68, 166 | L16996, AF161856 |
| C. muris (432) | 320, 112 | None | 42, 224, 166 | AF093498, AF093498, AF093497 |
| C. andersoni (431) | 319, 112 | None | 265, 166 | AF093496, L19069 |
| C. felis (455) | 239, 104, 112 | 50, 405 | 221, 68, 166 | AF087577 |
| C. baileyi (428) | 212, 104, 112 | 84, 344 | 262, 166 | L19068, AF093495 |
| C. meleagridis (434) | 47, 171, 104, 112 | None | 200, 68, 166 | AF112574 |
| C. serpentis (430) | 318, 112 | None | 264, 166 | AF093502a |
| C. wrairi (435) | 219, 104, 112 | None | 201, 68, 166 | AF115378 |
| Cryptosporidium pig (435) | 219, 104, 112 | None | 201, 68, 166 | AF108861 |
| Cryptosporidium desert monitor (432) | 216, 108, 112 | None | 198, 68, 166 | AF112573 |
| Cryptosporidium mouse (439) | 48, 175, 104, 112 | None | 205, 68, 166 | AF108863 |
| Cryptosporidium ferret (438) | 48, 174, 103, 113 | None | 204, 68, 166 | AF112572 |
| Cryptosporidium dog (429) | 213, 104, 112 | None | 195, 68, 166 | AF112576 |
| Cryptosporidium koala (436) | 220, 104, 112 | None | 202, 68, 166 | AF108860 |
| Cryptosporidium kangaroo (436) | 220, 104, 112 | None | 202, 68, 166 | AF112570 |
| Cryptosporidium monkey (436) | 220, 104, 112 | None | 202, 68, 166 | AF112569 |
| Cryptosporidium bear (432) | 216, 104, 112 | None | 87, 111, 68, 166 | AF247535 |