FIG. 1.

Methanol metabolism in B. methanolicus. Methanol is initially oxidized to formaldehyde, which may be dissimilated to CO₂ or assimilated to pyruvate via the RuMP pathway. From pyruvate, the carbon flow is split between entering the TCA cycle or conversion to aspartate and its related amino acids. Abbreviations: TCA, tricarboxylic acid cycle; RuMP, ribulose monophosphate pathway; PYR, pyruvate; ASP, aspartate; OAA, oxaloacetate; OG, 2-oxoglutarate; GLU, glutamate; LYS, l-lysine; CoA, coenzyme A. Enzymes: 1, pyruvate dehydrogenase, PDH; 2, citrate synthase, CS; 3, pyruvate carboxylase, PC; 4, glutamate:oxaloacetate aminotransferase, GOT; 5, aconitase, ACN; 6, isocitrate dehydrogenase, IDH; 7, glutamate synthase, GOGAT; 8, 2-oxoglutarate dehydrogenase, OGDC.