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Time course of CS activities of B. methanolicus mutant NCS-L-7 and B. methanolicus wild-type NOA2. Cells were grown in shake flasks at 50°C in MVcMY medium supplemented with glutamate (1 g per liter), and samples were taken throughout the growth phase for OD500 and CS activity measurements. Mean values of duplicate experiments ± 1 standard deviation are shown. Growth: NCS-L-7, □; NOA2, ▪. CS specific activity: NOA2, •; NCS-L-7, ○.
