FIG. 3.

Fed-batch growth and amino acid secretion by B. methanolicus strains with glutamate feeding. Cells were grown in 14-liter bioreactors at 50°C in MVcMY medium and with methanol, glutamate, and methionine feeding. Samples were removed throughout the growth phase for OD₅₀₀ measurements and analyzed for amino acid content. (A) NCS-L-7 (with glutamate and methionine feeding), (B) NOA2 (with glutamate and methionine feeding), (C) NOA2 (without glutamate or methionine feeding). Glutamate, ⋄; l-lysine, □; aspartate, ▵; cell dry weight, ▪.