TABLE 2.

Enzyme activities in wild-type (MGA3) and l-lysine-overproducing mutant (13A52-8A66) B. methanolicus strains^a

Strain	Activity [nmol min−1 (mg of protein)−1]
	PC	Pyruvate dehydrogenase	Glutamate: oxaloacetate aminotransferase	CS	2-Oxoglutarate dehydrogenase	Phosphoenolpyruvate carboxylase
MGA3	161 ± 45	33 ± 4.4	142 ± 7.3	126 ± 5.6	0.96 ± 0.11	ND
13A52-8A66	535 ± 22	5.5 ± 0.67	55 ± 8.1	292 ± 28	7.3 ± 0.61	ND

^aCells were grown in shake flasks in MVcMY medium (see text). All measurements were made at 50°C in crude extracts prepared from cells harvested in stationary phase. All experiments were performed in duplicate or triplicate, and values are reported as means ± 1 standard deviation. ND, not detected.