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Intracellular metabolite concentrations in B. methanolicus MGA3 and mutant 13A52-8A66a
| Strain | Intracellular metabolite concn (mM)
|
|||||
|---|---|---|---|---|---|---|
| Pyruvate | Oxaloacetate | 2-Oxoglutarate | Aspartate | Glutamate | l-Lysine | |
| MGA3 | 3.2 | 449 | 2.7 | 8.1 | 87.7 | 2.3 |
| 13A52-8A66 | 0.6 | 448 | 0.6 | 5.0 | 428 | 66.8 |
Cells were grown in MVcMY medium in 1-liter baffled shake flasks at 50°C until stationary phase, and crude extracts were prepared and measured for metabolite concentrations as described in the text.
