FIG. 3.
Effect of CSP overproduction on L. plantarum growth at optimal temperature. (A) Cultures of the control strain (dotted line, open circles) and the CSP-overproducing strains (solid lines; closed triangles, CspC; closed circles, CspL; closed diamonds, CspP) were grown at 27°C after inoculation with stationary-phase precultures. Nisin induction (25 ng ml−1) was started 1 h after inoculation, and growth was monitored for 8 to 9 h. (B) Growth of control (dotted lines, open symbols) and CspC-overproducing strains (solid lines, closed symbols) upon induction with various nisin concentrations (0 ng ml−1 [triangles], 7 ng ml−1 [squares], and 25 ng ml−1 [circles]). Error bars represent the standard deviations from the means (n = 3).