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. 2000 Jun 13;97(13):7184–7189. doi: 10.1073/pnas.140202297

Figure 4.

Figure 4

(A) Analysis of immunoaffinity-purified RNAP IIO and RNAP IIA by SDS/PAGE and silver staining. (B) Comparative immunoblot analysis of immunoaffinity-purified RNAP IIO and RNAP IIA. The large RPB1 subunit of RNAP II was analyzed by using antibodies H5 (Babco) and N-20 (Santa Cruz Biotechnologies) described in the legend of Fig. 2. (C) High levels of transcription activation in human NEs requires CTD-phosphorylated RNAP IIO. NE[ΔNC2β] lacking RNAP IIO (Fig. 2) was supplemented with equal amounts (RPB6 content) of RNAP II purified from HeLa nuclear pellet (lane 3) containing IIO, IIA, and IIB forms (Fig. 3D), immunoaffinity-purified RNAP IIA (f:RNAP IIA, lane 4), and immunoaffinity-purified RNAP IIO (f:RNAP IIO, lane 5).