TABLE 1.
Primers designed for cloning of putative Thermus competence genes
| Plasmid generated | Primer designation | Primer sequence (5′-3′)a |
|---|---|---|
| pAF1699 | orf1699-BI | CCT CTT CGG ATC CGT CAT TAG |
| orf1699-EI | CGG CTG GAA TTC CCA CAA TGA G | |
| pAF1698 | orf1698-EI | GTT GCA GAA TTC CAA GGC TGC |
| orf1698-BI | GAT ACA TCC GGA TCC CGT TTG TC | |
| pJR1 | orf1644-EI | CGT GGC GGA ATT CGC GGA TGG |
| orf1646-XI | GAC GAG CTC TAG ACC CGC GTC | |
| pJR2 | orf1695-XI | CGG ATG CTC TAG ACC TCG GTG |
| orf1697-EI | GCA GCC TTG GAA TTC TGC AAC CC |
a
Modified base pairs are indicated in bold.