Figure 2.

Dependence of lac and btuB RNA binding on concentration of 30S ribosomal subunits. Ribosome binding is measured as the relative amount of the T256 product, expressed as a percentage of the major primer extension products. Binding of unwashed (open symbols) and high-salt-washed (filled symbols) ribosomes to lac (circles) and btuB (squares) RNA. Binding of unwashed ribosomes to btuB RNA in the presence of 5 μM Ado-Cbl is indicated (triangles). Curves are fit to a hyperbolic binding process.