TABLE 3.
M. elsdenii strain characteristics
| Straina | Animal or sample | V3 sequence similarityb to RLC-1 (%) | Growth on lactate (OD620) | MIC (μg/ml)c
|
|||
|---|---|---|---|---|---|---|---|
| Tet | Ctc | Otc | Dox | ||||
| LC-1T | Ovine | 100 | 1.75 | 4 | 4-8 | 8-16 | 1 |
| B159 | Bovine | ND | 1.7 | 4 | 4-8 | 8-16 | 1 |
| T81 | Ovine | ND | 1.75 | 4 | 2-4 | 8-16 | 1-2 |
| 2-9 | Swine cecal contents (farm A) | 98.2 | 1.8 | 128 | 256 | 256 | 64 |
| 4-13 | Swine cecal contents (farm A) | 99.4 | 1.7 | 128 | >256 | 256 | 64 |
| 7-11 | Swine cecal contents (farm A) | 98.9 | 1.8 | 128 | >256 | 256 | 64 |
| 7-12 | Swine cecal contents (farm A) | 98.9 | 1.6 | 128 | >256 | 256 | 64 |
| 14-14 | Swine cecal tissues (farm B) | 99.4 | 1.85 | 256 | >256 | >256 | 64 |
| 15-5 | Swine cecal tissues (farm B) | 98.9 | 1.8 | 128 | 256 | 256 | 64 |
| 19-3 | Swine cecal tissues (farm B) | 98.9 | 1.55 | 128 | 256 | 256 | 64 |
| 20-11 | Swine cecal tissues (farm B) | 98.9 | 1.4 | 128 | >256 | 256 | 64 |
| Bacillus fragilis ATCC 25285 (TcS) | 1-2 | 4 | 8-16 | 1 | |||
| Bacillus thetaiotaomicron ATCC 29741 (Tcr) | 64 | 128 | 64 | 32-64 | |||
a
M. elsdenii strains LC-1T, B159, and T81 were obtained from stock cultures. Additional M. elsdenii strains were isolated in these studies. Bacteroides strains were obtained from the American Type Culture Collection for use as reference strains in tetracycline MIC assays (41).
b
Percent sequence similarity with M. elsdenii LC-1T (U95027) in the 16S rDNA V3 region. ND, not determined.
c
Tetracycline (Tet), chlortetracycline (Ctc), oxytetracycline (Otc), and doxycycline (Dox) concentrations expressed per milliliter of medium.