TABLE 1.
Bacterial strains and plasmids used in this study
| Bacterial strain(s) or plasmid | Characteristics | Reference or source |
|---|---|---|
| Bacillus sp. strain HIL Y-85,54728 TT | Producer strain of mersacidin | 8 |
| M. luteus ATCC4698 | Indicator strain | ATCC strain collection |
| S. pyogenes I-12662 | Indicator strain | This study |
| S. carnosus TM300 | Cloning host | 27 |
| E. coli JM 83 | Cloning host | 31 |
| E. coli 71/18, JM 109, HMS 174 | Cloning hosts | 26 |
| E. coli 71/18 BHM mutS | Cloning host for site directed mutagenesis | 32 |
| pTV0 | Temperature-sensitive gram-positive vector, CmR | 1 |
| pTV0[MCS] | pTV0 carrying a multiple cloning site | 15 |
| pS16I14 | pTV0 carrying S16ImrsA in its PvuII site | This study |
| pE17A14 | pTV0 carrying E17AmrsA in its PvuII site | This study |
| pF3L14 | pTV0[MCS] carrying F3LmrsA | This study |