TABLE 2.

Primers used in this study

Primer	Sequencea	Product or function
PTV01	CACAAAAAACAGGTACAAGAAAAAC	442-bp fragment of pTV0[MCS]
PTV02	ACCCCTCTTTCCATGTATTCACT
NPTV02	AAGGAGTTAAGTATTATGACTGGG	818-bp product in absence of the plasmid
Primer 5′	GGGTATATGCGTATAAACTTATG
mrsAmutS16I	5′d(GTTTGTACTCTAACTTTAGAATGTATTTGTTAATT)3′	Mutagenesis
mrsAmutE17A	5′d(TACTCTAACTTCTGCATGTATTTGTTAATTTG)3′	Mutagenesis
mrsAmutF3L	5′d(GAAGCAGCATGTACGCTTACATTGCCTGG)3′	Mutagenesis

^aMismatches of the oligonucleotides used for mutagenesis are underlined.