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. 2003 Jul;69(7):4219–4226. doi: 10.1128/AEM.69.7.4219-4226.2003

TABLE 1.

Bacterial strains and plasmids

Strain or plasmid Relevant properties Source or reference
Strains
    E. coli XL1-Blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM15 Tn10(Tcr)] Stratagene Ltd.
    E. coli ANCC22 phoR and creC mutations 29
    E. coli ANCL1 PhoB− 29
    E. coli VJS3051 ΔnarQ251::Tn10d (Tcr) ΔnarX242 zch-2084::Ω-Cmr φ(fdnG-lacZ) 40
    E. coli VJS3081 Δ(lac-argF)U169 λφ(fdnG-lacZ) narL215::Tn10 41
    B. infantis UCC 35624 Wild-type human isolate UCC Culture Collectiona
Plasmids
    pBluescript KS− Apr αlacZb Stratagene Ltd.
    pWSK29 Apr αlacZ,b low copy number 56
a

UCC, National University of Ireland, Cork, Ireland.

b

αlacZ is the portion of the lacZ gene that provides α-complementation for blue and white color selection of recombinant phagemids.