TABLE 1.
RT-PCR primers evaluated for the detection of caliciviruses in calf fecal samples
| Primer pair | Sequence (5′→3′) | Sense | Target virus(es)b | Source or reference |
|---|---|---|---|---|
| NV36 | 5′-ATA AAA GTT GGC ATG AAC A | + | NLV-GI | Wang et al. (27) |
| NV35 | 5′-CTT GTT GGT TTG AGG CCA TAT | − | NLV-GI | Wang et al. (27) |
| P290 | 5′-GAT TAC TCC AAG TGG GAC TCC AC | + | NLV, SLV | Jiang et al. (11) |
| P289 | 5′-TGA CAA TGT AAT CAT CAC CAT A | − | NLV, SLV | Jiang et al. (11) |
| JV12 | 5′-ATA CCA CTA TGA TGC AGA TTA | + | BEC, NLV | Vinjé et al. (26) |
| JV13 | 5′-TCA TCA TCA CCA TAG AAA GAG | − | BEC, NLV | Vinjé et al. (26) |
| J11U | 5′-CCA TCA ACC ATT GGA TTT TGA C | + | NLV-GIII | H. Tsunemitsua |
| J11L | 5′-ACC TGG CGG CGA AGC TCA ATT G | − | NLV-GIII | H. Tsunemitsua |
| J21U | 5′-AAC CAT TGG ATA TTA ACC CTG TG | + | NLV-GIII | H. Tsunemitsu |
| J21L | 5′-GAC CAT CTA CCT GCT GCT TCA | − | NLV-GIII | H. Tsunemitsu |
| BEC-POL5′ | 5′-TAT GAG CCA GCC TAC CTT GG | + | NLV-GIII | K. O. Chang and L. J. Saif, unpublished data |
| BEC-POL3′ | 5′-ACC TGG GAC GTG CAT GGG A | − | NLV-GIII | K. O. Chang and L. J. Saif, unpublished data |
| NBU-F | 5′-TTT CTA ACY TAT GGG GAY GAY G | + | BEC-NB-like | J. R. Smiley and L. J. Saif |
| NBU-R | 5′-GTC ACT CAT GTT TCC TTC TCT AAT | − | BEC-NB-like | J. R. Smiley and L. J. Saif |
| CBECU-F | 5′-AGT TAY TTT TCC TTY TAY GGB GA | + | NLV-GIII | J. R. Smiley and L. J. Saif |
| CBECU-R | 5′-AGT GTC TCT GTC AGT CAT CTT CAT | − | NLV-GIII | J. R. Smiley and L. J. Saif |
a
Primer sequences based on BECs identified in Japanese dairy calves genetically related to BEC/Jena and NA-2 were kindly shared by Hiroshi Tsunemitsu, Japanese National Institute of Health-Scichinohe Research Unit, Shichinohe, Aomori, Japan.
b
NLV-GIII, “classic” NLV-like BECs; BEC-NB-like, NB-related BECs.